In general, it is preferred when large amounts of blood replacement fluids are needed or when low blood pressure or other problems prevent the use of a suitable blood vessel for intravenous injection. Nelson from the laboratories of physiological chemistry, iowa state college, ames received for publication, may 5, 1932. Subcutaneous compared with intraperitoneal ketaminexylazin. Intraperitoneal injection synonyms, intraperitoneal. Intraperitoneal injection is a common method of administering drugs to rodents. All animal users performing ip injections in rodents must. Intraperitoneal injection is a common route for parenteral administration of drugs in rodents. The drug concentrations in peritoneal solid cancer tissue and suspended free cancer cells were also significantly higher for a longer time in the intraperitoneal injection group than in the intravenous injection group. Optimization of intraperitoneal injection anesthesia in mice. Blood lactate dynamics in awake and anaesthetized mice after. We similarly found an error in the intraperitoneal injection of mice and. Sevenweekold mice received an intraperitoneal ip injection of vehicle physiological saline or enu at a dose of 50, 100, 200, 400, or 600 mgkg 5 mice per dose. Intraperitoneal ip injection in rats and mice sop ubc animal. Intraperitoneal injection definition of intraperitoneal.
Pharmacokinetic advantage of intraperitoneal injection of. Tail handling and scruff handling of mice one hand only duration. In addition, fewer female mice achieved surgical anesthesia, suggesting a narrow therapeutic window for intraperitoneal kx in female mice. Errors in the technique of intraperitoneal injection of mice. Effects of administration route, dietary condition, and blood.
The intramuscular technique requires more skill than does subcutaneous. Intraperitoneal injection the administration of material into the peritoneal cavity is frequently performed in mice. To determine glucose tolerance, we determined the blood glucose auc during the glucose tolerance test. Pull back on the plunger to ensure negative pressure prior to injecting. Synonyms for intraperitoneal injection in free thesaurus. Feb 26, 2016 see more video protocols, please visit restrain the mouse by the scruff method. Intraperitoneal injection an overview sciencedirect topics. Oct 14, 2015 appswe and wild type appwt mice at 12 months of age were given a daily intraperitoneal injection with the tatbdnf peptide pep, 2. Burrows the research institute, the royal cancer hospital free, london. Evaluation of a novel technique for intraperitoneal. Data available for intraperitoneal injection of 70% or 100% ethanol in mice suggests 4560s 75th percentile to achieve lorr and 3. Edta was found to be nontoxic to the cell lines by.
However, pristane also induces plasmacytomas and an erosive arthritis resembling rheumatoid arthritis in balbc mice, probably as a consequence of enhanced interleukin 6 production. If there is negative pressure, proceed with the injection. In conclusion, intraperitoneal injection of omt could beneficially decrease the cciinduced mechanical allodynia and thermal hyperalgesia, antagonize the effect of nmda, synergistic enhance the effect of mk801 and infenprodil, led to a marked decrease in nmda nr2b, perk and pcreb induced by cci in the spinal cord in mice. Pdf intraperitoneal injection is a common technique that safely delivers a. The aim of this technique is to administer material into the space surrounding the abdominal organs, avoiding injection directly into any organ.
A serious consequence associated with this technique, however, is the puncture of vital organs such. Effect of intraperitoneal injection volume and antibody protein dose on the pharmacokinetics of intraperitoneally administered igg2ac murine monoclonal antibody in the rat1 jeffrey s. Health, general cancer chemotherapy health aspects injections, intraperitoneal research intraperitoneal injections ovarian cancer drug therapy risk factors. The intramuscular administration is not recommended, as the muscle of the mouse is too small. Method of intraperitoneal injection in wistar rat duration. Effect of intraperitoneal injection volume and antibody. Conclusion these data indicate that a single intraperitoneal injection of ketamine at subanaesthetic and anaesthetic doses does not impair working memory, reference memory or neurodegeneration in adult mice, but an intermediate dose of ketamine produces transitory hyperlocomotion.
This is consistent with the trend of greater effect on the nervous system of. Considering the risks and safety of intraperitoneal injections. Intraperitoneal injection article about intraperitoneal. Considering the risks and safety of intraperitoneal injections article pdf available in lab animal 454. The final biodistribution pattern of nphdl 59fespio after intravenous and intraperitoneal application in mice looked similar. Intraperitoneal injection improves the uptake of nanoparticle. Mar 22, 2016 considering the risks and safety of intraperitoneal injections. The highest activity uptake was measured in the liver and spleen, followed by the femur, aorta, and in the peritoneal macrophages in the case of intraperitoneal injection.
The ld 50 of thymoquinone in rats was determined to be 57. Intraperitoneal ip injection is frequently reported to be as effective as intravenous iv injection. Get a printable copy pdf file of the complete article 285k, or click on a page image. At 24 h after injection, the intensity of free dye was 100 folds less than the dir.
Intraperitoneal injection in the mouse although widely used as a means of administering substances, particularly injectable anaesthetics, this is an inherently unreliable technique, since inadvertent injection of some material into the gut, abdominal fat and subcutaneous tissues is a relatively frequent occurrence. The laboratory mouse handbook of experimental animals usp. The mice were given a single subperitoneal injection of stz 300 l for routine feeding with the mother mice. Intraperitoneal definition of intraperitoneal by medical. The injection site should be in the lower left quadrant of the abdomen because vital organs are absent from this area.
In conclusion, surgical anesthesia of mice with subcutaneous kx k, 191. Only the tip of the needle should penetrate the abdominal wall, to prevent injection into the intestine waynforth, 1980. Understand the benefits and risks of intraperitoneal therapy. Improvised peritoneal dialysis in an 18monthold child with severe acute malnutrition and acute kidney injury a case report p02.
Retinal degeneration induced in adult mice by a single. Lactate treatment has shown a therapeutic potential for several neurological diseases, including alzheimers disease. Intraperitoneal injection is not a suitable administration. Mice mus musculus are small rodents that breed and sexually mature quickly, making them perfectly suited to generating large animal colonies for biological research. Media in category intraperitoneal injections the following 9 files are in this category, out of 9 total. Induction of lupusassociated autoantibodies in balbc mice.
Calculation of glucose dose for intraperitoneal glucose. Intraperitoneal ip injection in rats and mice sop page 2 of 6 70% isopropyl alcohol to disinfect top of multi. In this study, we performed intraperitoneal glucose tolerance tests in mice with dietinduced obesity and their lean controls, with glucose doses based on either the total body weight or the lean body mass of the animals. In this study, intraperitoneal ip injection was used to mimic the human administration route. Using 165 mice, 24 % received the ip injection in a place other than the peritoneal cavity. It is more often applied to animals than to humans. The icr newborn male mice were randomly selected for intraperitoneal injection of stz beyotime institute of biotechnology, shanghai, china. Abbot japan, tokyo, japan and sacrificed by exsanguination from aortic transection. Intraperitoneal xenografts of human epithelial ovarian cancer in nude mice bruce g. For all species, many different routes are available for administration of substances. In this study, we tested the ability of avidin, labeled through conjugation to radioactive biotin a b vitamin, to target intraperitoneal tumors.
Intraperitoneal injection in mice research at penn state. Intraperitoneal xenografts of human epithelial ovarian. Considering the risks and safety of intraperitoneal. Because it allows administering a larger volume with more radioactivity, we have investigated this route and the possibility of using it to circumvent the volume constraint we earlier experienced with pretargeting radiotherapy. Effect of intraperitoneal or intracerebroventricular. Understand the benefits and risks of intraperitoneal therapy for advanced ovarian cancer. Avidin targeting of intraperitoneal tumor xenografts jnci.
Dec 01, 1994 intraperitoneal injection of pristane 2,6,10,14 tetramethylpentadecane is a standard technique for obtaining monoclonal antibodyenriched ascitic fluid. The november 2016 issue of lab animal included an article by baek and colleagues 1 describing a novel technique for handling mice. The goals of the study were to find a safe intraperitoneal injection anesthesia protocol for mediumduration surgery in mice e. Intraperitoneal ip injection in rats and mice sop page 4 of 6 mouse rat 6. The laboratory mouse handbook of experimental animals. Evaluation of a novel technique for intraperitoneal injections in mice article pdf available in lab animal 4411. As seen in the clinical journal of oncology nursing by ons connect. Intraperitoneal injection in the mouse procedures with care. In a previous study, 14 mice were exposed to swcnts through intravenous iv injection to determine whether there were any adverse effects caused by swcnts on the mouse brain and also to find a safe application dosage if possible. Recommended needle sizes and injection sites for various species are indicated. Some sites, such as footpad injection of freunds complete adjuvant, intrasplenic injection and intra lymph node injection. Good practice administration routes and volumes for mice, rats, and rabbits are indicated in table 1. We use cookies to offer you a better experience, personalize content, tailor advertising, provide social media features, and better understand the use of our services.
Pdf evaluation of a novel technique for intraperitoneal injections. Using 165 mice, 24% received the ip injection in a place other than the peritoneal cavity. A single intraperitoneal injection of ketamine does not affe. Intraperitoneal ip injections should be made into the animals right abdominal quadrant so as to avoid penetrating the cecum on the left side. Restrain mice with their abdomen exposed uppermost and their head pointed. Results of intraperitoneal and oral administration of thymoquinone in mice are given in tables1a and 1b, respectively. For purposes of control, lard, olive oil and other fatty materials free from added carcinogenic compounds were injected into rats and mice in the same way. Intraperitoneal injection of lipopolysaccharide lps. Procedure summary and goal describes procedure for the administration of fluids or compounds into the abdominal cavity. Good practice administration routes and volumes for mice. Administration of substances to laboratory animals.
Taylor1,3 1systems immunity research institute, cardiff university school of medicine, tenovus building, heath park, cardiff. Pdf evaluation of a novel technique for intraperitoneal. Effective in vivo gene modification in mouse tissue. Intraperitoneal administration of a novel tatbdnf peptide. Those trained persons listed on an approved animal care committee protocol performing the procedure. Intraperitoneal injection is a common technique that safely delivers a substance into the peritoneal cavity but can induce high stress in animals. Free dir and healthy mice without colitis were served as control. Intraperitoneal injection is not always a suitable. The control group was injected with the same amount of pbs buffer.
Intraperitoneal injection ip, intravenous injection iv or anal. In order to optimize the administration of lactate for studies in mouse models, we compared blood lactate dynamics after intraperitoneal ip and subcutaneous sc injections. Here we present an overview of the methods used to deliver tamoxifen to mice. Safety studies of the intraperitoneal injection of e1a. Four tumor models one ovarian, one lung, and two colon were established in nude mice by intraperitoneal injection of cultured cancer cells. The most used method is intraperitoneal injection, because the amount of administered compound can be better controlled, but delivery by oral gavage is also possible. Intraperitoneal injection in mice simple pharmacology and facts of medicines. As compared to other mammalian species, mice are simple and inexpensive to maintain in the laboratory. Intraperitoneal injection of lipopolysaccharide induces. Pharmacokinetic advantage of intraperitoneal injection of docetaxel in the treatment for peritoneal dissemination of cancer in mice.
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